HFBoards - View Single Post - Could/Should the Habs get Patrick Marleau?

10-23-2003, 08:47 AM

Firstly, we must take 3 things into consideration:

(I) Difficulty or success in floral transformation with Arabidopsis

(II) Problems of selecting for the pSOUP vector whose trfA function provides the replicase function for pGreen (see below). Many C58-derived strains (including GV series) develop spontaneous resistance to tetracyclin. The solution is to co-transform both pSoup and pGreen and use kanamycin selection solely, rather than tet/kan.
A chemical transformation method is available in MolBiol.Net > Protocols > Transformations

(III) Backbone rearrangements, pGreenI was prone to recombination with E.coli genomic DNA - has anyone has problems with the same and pGreenII

10-23-2003, 08:47 AM	#5
Le Gross Pork Guest Country: Posts: n/a vCash:	Firstly, we must take 3 things into consideration: (I) Difficulty or success in floral transformation with Arabidopsis (II) Problems of selecting for the pSOUP vector whose trfA function provides the replicase function for pGreen (see below). Many C58-derived strains (including GV series) develop spontaneous resistance to tetracyclin. The solution is to co-transform both pSoup and pGreen and use kanamycin selection solely, rather than tet/kan. A chemical transformation method is available in MolBiol.Net > Protocols > Transformations (III) Backbone rearrangements, pGreenI was prone to recombination with E.coli genomic DNA - has anyone has problems with the same and pGreenII